Subset giving correct dataframe in R, but subsequent functions using old dataframe

I am subsetting a large dataframe into a smaller one in R which works fine.

$ExampBio<-read.delim(“ExampBio.txt”, header=TRUE, sep =”t”)
$ExBio9<-subset(ExampBio, Experiment %in% c(“9”))

I double click ExBio9 in the environment box and see the correct subset dataframe, but I’m having trouble making a boxplot from the subset dataframe and when I go to check the levels for “ExBio9” it returns all the levels in the original dataframe (boxplot is also using the old dataframe although I’ve specified “ExBio9”). Any help is appreciated.

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